Clathrin Adaptors Really Adapt
نویسنده
چکیده
The clathrin pathway is the principal route for receptor-mediated endocytosis and growth factor downregulation. Heterotetrameric clathrin adaptors directly link the clathrin coat with cargo transmembrane proteins that are sorted into coated pits and vesicles. A paper in this issue of Cell describes the atomic structure of the adaptor-protein 2 (AP-2) core, the portion that makes contacts with the membrane and cytosolic tails of cargo proteins.
منابع مشابه
MinireviewClathrin Adaptors Really Adapt
proteins into clathrin pathways. Because of their key The clathrin pathway is the principal route for receprole in linking sorted proteins with the clathrin coat, the tor-mediated endocytosis and growth factor downregassembly factors are now referred to as heterotetramulation. Heterotetrameric clathrin adaptors directly eric clathrin adaptors. link the clathrin coat with cargo transmembrane pro...
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Clathrin is a ubiquitous protein that mediates membrane traffic at many locations. To function, clathrin requires clathrin adaptors that link it to transmembrane protein cargo. In addition to this cargo selection function, many adaptors also play mechanistic roles in the formation of the transport carrier. However, the full spectrum of these mechanistic roles is poorly understood. Here we repor...
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The coat proteins of clathrin-coated vesicles (CCV) spontaneously self-assemble in vitro, but, in vivo, their self-assembly must be regulated. To determine whether phosphorylation might influence coat formation in the cell, the in vivo phosphorylation state of CCV coat proteins was analyzed. Individual components of the CCV coat were isolated by immunoprecipitation from Madin-Darby bovine kidne...
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متن کاملCytosol- and clathrin-dependent stimulation of endocytosis in vitro by purified adaptors
Using stage-specific assays for receptor-mediated endocytosis of transferrin (Tfn) into perforated A431 cells we show that purified adaptors stimulate coated pit assembly and ligand sequestration into deeply invaginated coated pits. Late events in endocytosis involving membrane fission and coated vesicle budding which lead to the internalization of Tfn are unaffected. AP2, plasma membrane adapt...
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ورودعنوان ژورنال:
- Cell
دوره 109 شماره
صفحات -
تاریخ انتشار 2002